Effects of wine making and ultrafine pulverization on the dissolution of Astragalus membranaceus in Angelica sinensis

Effects of wine making and ultrafine pulverization on the dissolution of Astragalus membranaceus in Angelica sinensis
Wang Aiwu 1耿军1 Wei Wei 2
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(1,        Shandong Provincial Hospital, Jinan 250021, China; 2. Shandong University of Traditional Chinese Medicine, Jinan 250014, China)
[Abstract] Objective: To compare the effects of wine system and ultrafine pulverization on the dissolution of Astragalus membranaceus in Angelica sinensis. Method: High pressure liquid chromatography. The column was ODS-C 18 column, the mobile phase was methanol-water glacial acetic acid (47:53:0.1), the detection wavelength was 280 nm, the flow rate was 0.8 ml ̇min -1 , and the column temperature was room temperature. RESULTS: The wine system had almost no effect on the dissolution of Astragalus membranaceus in Angelica sinensis. Ultrafine pulverization could increase the dissolution of Astragalus membranaceus. Conclusion: For the dissolution of Astragalus membranaceus in Angelica sinensis, the significance of wine system is large, and ultrafine pulverization is of great significance.
[Key words] wine system; Danggui San; Huang Wei
[Chinese figure class number] R282.710.2 [ Documentation code] B [Article ID] 0257-358X (2001) 04-0237-02
Angelica originated from the "Golden Chamber", which is composed of Angelica, Chuanxiong, Astragalus, Radix Paeoniae Alba, and Atractylodes. The whole body has the functions of nourishing the blood and strengthening the spleen and clearing the heat and curing the fetus. It is a commonly used prescription for traditional Chinese medicine gynecology. The original side is made of raw pieces, and served with wine. After the evolution of the generation, in addition to Atractylodes, the other four drugs were derived into wine products. There have been reports on the dissolution effect of ferulic acid and paeoniflorin in the compound [1] , and there is no research on the dissolution of astragalus. In view of the fact that Astragalus membranaceus is the main drug for the treatment of heat and fetus in Fangzhong, this paper compares the amount of dissolution of Astragalus membranaceus in wine system, aiming to comprehensively measure the significance of wine system. It is also reported that the ultrafine pulverization technology has unique advantages and broad application prospects in improving the processing technology of traditional Chinese medicine and improving the quality of solid preparations of traditional Chinese medicines [2] . This paper introduces this technology to study the dissolution of jaundice in Angelica sinensis. The influence, on the other hand, explores the significance of Angelica sinensis and its preparation.
1 instruments and materials
Instruments: Water 2690 High Performance Liquid Chromatograph and 996 Diode Array Detector, SQW-6 Ultrafine Crushing Mixer, SB 2200 Ultrasonic Generator (manufactured by Shanghai Bianxin Ultrasonic Co., Ltd.), Electronic Analytical Balance MET -TIER 240 (produced in Switzerland).
Pharmacopoeia and Reagents: Raw Angelica Powder and Wine Angelica Powder are provided by our Chinese Medicine Preparation Room. The superfine raw angelica powder is made by the Shandong Institute of Pharmaceutical Industry using ultrafine grinding technology. Astragalus reference substance is provided by China National Institute for the Control of Pharmaceutical and Biological Products. The reagent used, methanol is chromatographically pure, water is raw heavy distilled water, glacial acetic acid and ethanol are analytically pure.
2 experimental methods
2.1 Chromatographic conditions ODS-C 18 column (4.6mm × 150mm, 5? m), mobile phase is methanol-water-glacial acetic acid (47:53:0.1) detection wavelength is 280nm, flow rate is 0.8ml ̇min -1 , column The temperature is room temperature.
2.2 Preparation of the standard curve of Astragalus membranaceus accurately weigh the appropriate amount of Astragalus membranaceus, dissolve it in methanol and dilute it into a solution with a concentration of 40?g ̇ml -1 , and accurately draw the reference solution 0.63, 1.25, 2. 00, 2.50, 3.75 5.00 ml, diluted with methanol to 5 ml, shake well, respectively, take 10 μl of each of the above liquids, inject into the liquid chromatograph, record the chromatogram, and linearly regress the peak concentration Y to the injection concentration X, and obtain the regression equation. Y=30199.45X+5601.98, γ=0.9998, the reference substance of Astragalus membranaceus has a good linear relationship in the range of 0.05~0.40?g.
2.3 Preparation and determination of sample solution    1 g of each sample was taken, accurately weighed, 50 ml of 50% methanol was added, ultrasonically extracted for 20 min, and filtered. Take 1ml of filtrate, dilute to 10ml with 50% methanol, filter with 0.45 microporous membrane, draw 2ml to the injection valve, and record the peak area of ​​Astragalus according to the above chromatographic conditions. Calculate the content.
2.4 Precision, reproducibility and stability of the experimental method    The same reference solution was taken and measured in parallel for 5 times. The peak area was recorded and the RSD was 1.72%, indicating that the precision was good. Take 5 samples of the same batch and determine the RSD by 1.56% according to the sample measurement method, indicating good reproducibility. The same sample solution was taken at 0, 2, 4, 6, and 8 hr, and the peak area was basically unchanged, and the RSD was 1.6%.
3 experimental results
Take 5 samples of the same sample and operate according to the sample determination method. The results are shown in Table 1.
Table 1 sample of xanthine content
m/?g ̇ mg -1
Astragalus content (χ)                  average value
Wine system Angelica scattered 25.993 26.401 26.578 27.054 26.695 26.544
Health Angelica scattered 26.426 26.941 27.436 27.182 26.702 26.937
Super fine raw angelica scattered 31.472 31.530 32.093 32.125 31.350 31.714
It can be seen from Table 1 that the dissolution of Astragalus membranaceus is slightly reduced after the Angelica sinensis wine system, and the dissolution of Astragalus membranaceus is significantly increased after the ultrafine treatment.
4, discussion
Danggui Sanyuan is a raw medicine powder, that is, it is washed with alcohol. Later, the recipes of Angelica, Chuanxiong, Baiji and Huangqi wine will be taken into the medicine and washed with water. Some studies believe that the four medicines are slightly soluble in the total decoction. In addition, the dissolution of the other five components (reducing sugar, protein, volatile oil, ferulic acid, and peony) is lower than that of the bio-formulation [1] . The results of this study further suggest that Angelica sinensis uses the current wine product formula, which does not show that the dissolution of the compound ingredients in the body is a complicated process, and there is a certain gap between the in vitro test and the dissolution in the body. After the Chinese medicine is made into wine, the color and taste of the solution have indeed changed. The problem of the choice of wine products has yet to be pharmacologically and clinically verified.
The experimental results show that the dissolution of Astragalus membranaceus is significantly increased after the ultrafine pulverization of Angelica sinensis. This is consistent with the report that “the cells are fully exposed after the cell is broken, and the release rate and release amount of the drug will be large. The increase in amplitude" [3] is the result. Angelica sinensis contains a variety of active ingredients, and other factors such as paeoniflorin and ferulic acid are related to jaundice, which needs further study. Of course, the ultimate goal of Zui's ultrafine comminution is to improve bioavailability and efficacy, as evidenced by pharmacodynamic experiments.
[references]
[1]Lv Wenhai, Dong Wei. Comparison of Dissolution Effect of Danggui Powder with the Same Method of Using Wine[J].Journal of Shandong University of Traditional Chinese Medicine,1997,21(1):69.
[2]Wang Aiwu, Lu Wenhai, Yan Hui. Generalization and Prospect of Ultrafine Crushing in the Production of Traditional Chinese Medicine[J].Shizhen Guo Ma Guo Guo,2000,11(7):669.
[3] Guo Qi, Du Xiaomin. Effect of cell-based micro-pulverization on absorption in vivo[J]. Chinese Patent Medicine, 1999, 21(11): 601.

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