Key Techniques for Artificial Breeding of Smilax glauca
1. The broodstock is selected for the breeding of the fleas. The female quail should choose a body length of 35 cm or more, weigh 50-150 g, there is a zona pellucida in the abdomen, the genital hole is red and swollen, and the oocytes are the best; The length of the election is 55 cm or more, and the weight is more than 150 grams. There are blood-streaked markings on the ventral surface, and the abdomen can be squeezed out of the transparent fluid. Microscopic examination can reveal the type of sperm. It is advisable to use 2-3 females with one male. 2. In the case of oxytocosis, in general, inflating, increasing the amount of water exchange, adjusting the breeding density, and providing high-quality bait, etc. Subjects may also have a one-time injection of LRH-A. Females should receive 10 micrograms per 50 grams of body weight. Males should receive 15-25 milligrams per tail. Males should be given intrapleural injections 24 hours earlier than females. At the time of injection, the depth should not exceed 0.5 cm and the injection volume should not exceed 1 ml. After injection, put it into a cement pool or cage for raising. The water depth is 20-30 cm and the water is changed once a day. When the water temperature is 25°C, after 36 hours, the female's abdomen is touched, and when the eggs are released, artificial insemination can be performed. 3, artificial insemination with one hand to hold the front of the female tendon with a towel, the other hand to squeeze the abdomen back, in case of cloacal clogs, cloaca can be cut open, and then squeezing eggs, the extruded eggs into the disinfection of the cylinder or basin in. Kill the selected males, cut the testes, grind the testicles, wrap the cut testes with a 100-mesh sieve, squeeze the semen into the egg container, and stir for 5 minutes. Wash the testicles with blood to remove residuals and blood stains. Fertilized eggs are incubated in controlled temperature incubators or indoors. Regardless of the number of eggs, at least two or more males should be inseminated. 4, temperature control hatching water depth of about 10 cm is appropriate, the midway should often change the water, but the temperature should not exceed 4 °C. Embryos consume different amounts of oxygen in different stages, and the more they consume oxygen in the later stages, the oxygenator should be used to increase oxygen. The incubation time is closely related to the water temperature, and the higher the water temperature, the shorter the incubation time. If 22°C requires 288 hours for emergence, it will only take 134 hours at 26°C, and it will be shorter if the water temperature reaches 30°C. 5. For the cultivation of fry, cement tanks should be used. The water depth should be 30-40 cm. The upper edge should be more than 20 cm above the water surface, and the bottom should be at least 20 cm thick. A small amount of manure water is suitably applied in the pond for silkworm breeding, and aquatic plants such as water hyacinth are stocked on the water surface. The stocking density of fry is about 200 per square meter. The open bait is mainly silkworm, but it can also be fed with zooplankton or fish pulp, egg yolk powder, etc. Avoid large and small mixed ponds to avoid mutual killing. Often add new water, 1-2 months later, when the seedlings grow to about 10 centimeters, they can be moved into a breeding pond. 6, disease prevention and control seedlings rarely disease, but if poor management or bad environment, prone to water blight, coma and so on. Saprolegnia can be eliminated by soaking in 3%-4% saline for 3-4 minutes. In the hot season, shading and cooling can be used to change the pool water to prevent coma. Super Absorbent Bath Mat,Bath Mat,Diatomite Health Hot Bath Mat,Non Slip Bath Mat Dongguan Vanilla Bioengineering Co., Ltd. , https://www.healthecigarette.de