Protein concentration technology
(1) Concentrated gel concentration method Concentrated gel is an organic polymer with a polymer network structure and has strong water absorption performance. Each gram of dry glue can absorb 120ml ~ 150ml. It can absorb low molecular weight substances such as water, glucose, sucrose, inorganic salts, etc., and is suitable for concentrating biological macromolecular substances above 10 000 molecular weight. After concentration, the protein recovery rate can reach 80% to 90%. It is more convenient than concentrated glue and can be directly added to the concentrated solution. It must be noted that the pH of the concentrated solution should be greater than the isoelectric point of the concentrated material, otherwise cation exchange will occur on the surface of the concentrated gel, affecting the recovery rate of the concentrated material. (6) Dialysis bag concentration method The use of dialysis bags to concentrate protein solution is the most widely used one. Place the protein solution to be concentrated in a dialysis bag (no dialysis bag can be replaced with cellophane), ligation, and polymer (6 000-12 000) polymers such as polyethylene glycol (carbon wax), polyvinylpyrrole, alkanone, etc. Sucrose can be sprinkled outside the dialysis bag. The water absorbing agent may also be formulated into a solution having a concentration of 30% to 40%, and the dialysis bag containing the protein solution may be placed. After the water absorbing agent is used, it can be placed in a thermostat for drying or natural drying and can be reused. The Urine Reagent Strip Rapid Test is based on a double sequential enzyme reaction. One enzyme, glucose oxidase, catalyzes the formation of glucomic acid and hydrogen peroxide from the oxidation of glucose. A second enzyme, peroxidase, catalyzes the reaction of hydrogen peroxide with potassium iodide chromogen to oxidize the chromogen to colors ranging from blue-green to greenish-brown through brown and dark brown. Ketone Test,Urine Rapid Test Strips,Animal Test Strip,Test Strips Diabetes Weight Loss Changchun Medicon Technology Development Co., Ltd , https://www.jlmdk.com
(B) freeze-drying concentration method This is a better way to concentrate protein, which makes the protein less susceptible to denaturation, while maintaining the inherent components of the protein. It is directly sublimated in the frozen state to remove moisture. Specifically, the protein solution is frozen at a low temperature and then placed in a desiccator (a desiccant such as NaOH, CaCl2, and silica gel is contained in the desiccator). Closed, quickly evacuated, and maintained in an evacuated state. A dry powder containing the protein can be obtained in a few hours. The dried protein is easy to store and can be used in any concentration when applied. Freeze drying can also be carried out using a lyophilizer.
(3) Drying and Concentrating Method The protein solution is placed in a dialysis bag and placed under an electric fan. This method is simple, but slow, and the temperature should not be too high, it is best not to exceed 15 °C.
(4) Ultrafiltration membrane concentration method This method uses a microporous cellulose membrane to filter out water by high pressure, and the protein remains on the membrane to achieve concentration. There are two ways to concentrate: one is to insert the cellulose acetate membrane into the high pressure filter and filter under constant stirring; the other is to put the protein solution into the dialysis bag and built in the vent of the vacuum dryer, negative The air is pumped to make the liquid in the bag ooze out.
(5) Gel Concentration A gel with a small pore size, such as Sephadex G25 or G50, is used to directly add the gel to the protein solution. The amount of dry glue required is weighed according to the amount of water absorbed by the dry glue and the multiple of the protein solution to be concentrated. After being placed in a refrigerator, the gel particles are absorbed by water and then removed by centrifugation.
The test area of the Urine Reagent Strip Rapid Test is specific for glucose; no substance excreted in urine other than glucose is known to give a positive result.Approximately 100 mg/dl glucose in urine is detectable.
The Urine Reagent Strip Rapid Test is based on the protein error-of-indicator principle. At a constant pH, the development of any green color is due to the presence of protein. Colors range from yellow for a Negative reaction, to yellow-green and green to blue-green for a Positive reaction.