Mushrooms may appear during the cultivation of *Agrocybe aegypti*, and it's essential to take several precautions to avoid contamination of the bags. First, the cottonseed husks should be prepared and fermented 24 hours in advance to allow the temperature of the substrate to rise to 60–70°C. This high temperature helps eliminate harmful bacteria and ensures a clean growing environment.
Next, after mixing the ingredients with the bran, the spices are placed into the bags and sterilized on the stove for no more than six hours. This step is crucial to prevent the medium from becoming rancid or contaminated during the process.
Then, the sterilization process must be carried out at a temperature above 100°C for a full 24 hours. It’s important not to interrupt the process by adding cold water or stopping the heat prematurely, as this could lead to incomplete sterilization and bacterial growth.
After sterilization, handling the bags should be done with care to avoid any damage that might introduce contaminants. The bags should remain intact throughout the entire process.
When it comes to inoculation, it's best to choose a sunny night and strictly follow aseptic techniques. This reduces the risk of unwanted bacteria or pests entering the system. All equipment and tools should be sterilized before use, and the person handling the bags should wear gloves and maintain a clean workspace.
The temperature during the culture phase should be maintained between 23–26°C. Proper moisture levels, adequate ventilation, and a dark environment are also essential for healthy mushroom development. These conditions help the mycelium grow without stress or contamination.
Regular inspections should be conducted every seven days. If any signs of bacterial or pest contamination are detected, immediate action should be taken, such as isolating the affected bags or applying appropriate treatments to prevent the spread.
By following these detailed steps, the risk of contamination can be significantly reduced, leading to a successful and productive mushroom cultivation process. Each stage plays a vital role in ensuring the quality and yield of the final product.
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