Mushrooms may appear during the cultivation of *Agrocybe aegypti*, and it's essential to take proper precautions to avoid contamination of the growing bags. To ensure a successful and clean cultivation process, several key steps should be followed carefully. First, the cottonseed husks should be prepared and fermented 24 hours in advance. This allows the substrate temperature to rise to between 60–70°C, which helps eliminate harmful bacteria and ensures a cleaner environment for mushroom growth. Second, after mixing the ingredients with the bran, the spices should be placed into the bags and sterilized on a stove for no more than six hours. This prevents the matrix from becoming rancid or spoiled due to prolonged exposure to heat or improper conditions. Third, during the sterilization process, maintain a temperature above 100°C for at least 24 hours. It is crucial not to stop the heating process midway, add cold water, or allow the bags to cool down too quickly. A continuous and thorough sterilization is necessary to kill all potential contaminants. Fourth, after sterilization, handle the bags with care to avoid any damage. Even minor punctures or tears can introduce unwanted microorganisms, leading to contamination. Fifth, choose a sunny and calm night for inoculation. Strict aseptic techniques must be followed throughout the process to prevent bacterial or fungal entry. This includes using sterile tools, wearing clean gloves, and working in a controlled environment. Sixth, once the bags are inoculated, maintain the temperature between 23–26°C. Ensure proper moisture levels, good ventilation, and a dark environment to support healthy mycelium development. Lastly, perform regular inspections every seven days. If any signs of bacterial or pest contamination are detected, treat the affected areas immediately to prevent the spread of contaminants. Early detection and action are key to maintaining a successful crop. By following these detailed steps, you can significantly reduce the risk of contamination and improve the overall success rate of your *Agrocybe aegypti* cultivation.

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