Technical Features and Selection Guide for Lead-tech CO2 Incubator in Australia
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Abstract: This article introduces the technical characteristics of the six aspects of the Australian Lead-tech CO2 incubator, which has some common features of the CO2 incubator and also has some unique advantages of the company. Hope this article can provide users with some basic knowledge of CO2 incubator and a comprehensive understanding of the Australian Lead-tech CO2 incubator.
The Lead-tech CO2 incubator in Australia precisely controls the incubator temperature and CO 2 concentration through a microcomputer fuzzy PID controller to simulate three basic variables related to the in vivo environment: stable CO2 level, temperature and relative humidity. Create an environment that will make cells, tissues, and bacteria grow better: such as constant pH (pH: 7.2-7.4), stable temperature (37 ° C), high relative humidity (95%), stable CO2 Horizontal (5%) environmental conditions have improved the culture success rate and efficiency of biological cells, tissues and the like. The C02 incubator has become one of the most commonly used routine instruments in the laboratory and is widely used in medical, immunology, genetics, microbiology, agricultural science, pharmaceutical research and production. In the cell culture of the antibody, for diagnosis and treatment. Cell culture in toxicity test to identify drug materials; cell culture of virus to separate and diagnose; culture of cancer cells for analysis and treatment; cell culture of enzymes for drug production, food production, etc. Wait. Select new varieties of high-yield, high-quality, stress-resistant plants and animals; explore new drugs, vaccines and medical genes; develop protein engineering.
1. Basic characteristics and main features of Australia Lead-tech CO2 incubator
1.1 Basic features: (1) Heating method: air jacket type, water jacket type; (2) Sensor type: heat conduction type, infrared light; (3) gas type: single gas CO2; (4) effective capacity of box room: 90 , 160, 250L; (5) temperature range of the box RT + 5 ~ 65 ° C; (6) CO2 density range 0 ~ 20%;
1.2 Main features:
(1) Temperature control system: Microcomputer fuzzy PID control is adopted. The so-called fuzzy PID controller, which uses fuzzy logic algorithm and optimizes the proportional, integral and differential PID control according to certain fuzzy rules, in order to achieve better control effect. The conventional PID controller has simple structure, good stability, reliable operation and convenient adjustment. However, in a system with nonlinear time-varying and large hysteresis, the control effect is not ideal, and the setting of parameters is also a difficult task. For the user, the fuzzy PID controller does not need self-tuning, and can automatically adjust the PID value according to the change of the environment to achieve the optimal PID parameters.
(2) CO2 sensing system: Lead-tech carbon dioxide incubator C02 concentration detection can be measured by two kinds of control systems - infrared sensor (IR) and heat conduction sensor (TCD). The thermal conductivity sensor (TCD) monitors the CO2 concentration by measuring the change in resistance between two thermistors (one regulator exposed to the outside of the chamber and the other placed inside the chamber). The working principle is based on the continuous measurement of the thermal conductivity of the inner cavity air. The low thermal conductivity of the input CO2 gas changes the thermal conductivity of the air in the cavity, which produces an electrical signal directly proportional to the CO2 concentration. One disadvantage of the TCD control system is that changes in temperature and relative humidity within the chamber can affect the accuracy of the sensor. In addition, it can corrode and easily drift at zero. Suitable for users who have long-term culture of cells. The infrared (TR) sensor measures the CO 2 concentration by using infrared rays as a medium, that is, absorption of infrared rays by CO 2 gas. Its linearity is high and its sensitivity is high. It can measure 0-20ppm. The infrared sensor (IR) system includes an infrared emitter and a sensor. When the CO2 in the tank absorbs part of the infrared light emitted by the transmitter, the sensor can detect the reduction of infrared rays. The amount of infrared light absorbed corresponds to the level of CO2 in the tank, so that the concentration of CO2 in the tank can be obtained. The IR system is not affected by changes in temperature and relative humidity, and is especially useful for cell cultures that require frequent opening of the incubator door. However, the particles in the box can reflect or partially absorb infrared rays, making the IR system sensitive to the amount of particles in the box. Therefore, the IR sensor is suitable for use in an incubator containing a HEPA high efficiency air filter. The appendix contains the specific configuration of each type of carbon dioxide for users to choose.
(3) Heating system: The CO2 incubator is divided into two types: water jacket type and air jacket type according to the heating method. Both heating systems are accurate and reliable, and they all have their own advantages and disadvantages. Water jacket heating maintains a constant temperature by enclosing the inner tank with a separate water jacket, so the user is required to fill a large amount of distilled water in the space of the tank wall when installing the incubator. The advantages of the water jacket type: because of the 4 weeks of water, and the specific heat capacity of the water is relatively high, it is a good thermal insulation material, which helps to evenly dissipate heat and avoid the formation of cold zone. When encountering power failure, water jacket The system can maintain the temperature accuracy and stability in the incubator for a long time (the temperature is kept constant for 3-4 times of the air-tight system), and the temperature uniformity is very good. It is beneficial for users who are not stable in the experimental environment (such as useful electrical restrictions, or frequent power outages) and need to maintain stable culture conditions for a long time. The gas-sleeve heating is to directly heat the inner tank through the heaters in the gas jacket of the tank, and the temperature in the incubator is balanced by the heat insulation system and the surface heat dissipating components. Advantages of air-sleeve type: It has the characteristics of quick heating and recovery of temperature than the water-type incubator. It is especially beneficial for short-term cultivation and the need for frequent switching of the door. In addition, the user has a gas-sleeve design than water. The sleeve is simpler (water jacket requires watering, emptying and cleaning of the tank, and often monitoring the operation of the tank).
(4) Relative humidity control: It is very important to control the relative humidity in the incubator. Maintain sufficient humidity level to ensure that the culture will not fail due to excessive drying. The Lead-tech carbon dioxide incubator adds a large-area humidifying water tray to the bottom of the inner tank to maximize the evaporation area and enhance the evaporation, so that the incubator can quickly reach the saturated humidity state.
(5) Alarm system: DSA dual safety early warning system: The equipment is equipped with electronic safety early warning device and independent temperature limiting device to effectively prevent sudden equipment failure caused by abnormal operation. It has upper and lower limit temperature alarms, automatically sets the temperature alarm, and automatically sets the CO2 concentration alarm. When the temperature and concentration deviate from the set value within a certain range (adjustable), it will alarm and remind the user to pay attention to the observation. External gas decompression device: Before connecting the high-pressure CO2 gas into the culture tank, the equipment should properly decompress and filter it to avoid the chaos caused by the high-pressure gas on the wind-guiding structure inside the box, and appropriately reduce the relative air vibration. To ensure a good culture environment.
(6) Pollution control: Carbon dioxide incubator pollution is a major factor leading to cell culture failure. Therefore, the main way to reduce and prevent pollution is to minimize the areas and surfaces where microorganisms can grow and take disinfection measures. From reducing the area and surface where microorganisms can grow: SUS304 stainless steel large rounded mirror inner liner is used to effectively reduce dirt residue and microbial growth on the inner wall, which is convenient for users to clean and maintain the equipment daily. In terms of disinfection measures: UV sterilization and 90 ° C high temperature and high humidity sterilization, in addition, all Lead-tech models have HEPA filters, which can filter the potential sources of pollution and particulate impurities, and can remove 99.99% of the filter. Particles above 0.3 μm.
2. Conclusion
At present, most laboratories use imported cell culture incubators. The reason is that the quality of imported products is relatively safe, but the defects of imported products are also obvious. First, after-sales service is not very convenient. Second, product prices and subsequent maintenance prices are expensive. However, with the improvement of domestic technology and the development of international trade, joint ventures such as Lead-tech Scientific Instruments Co., Ltd. have achieved unprecedented development. Lidetai (Shanghai) Scientific Instrument Co., Ltd. is the design and development of high-end laboratory basic equipment by the advanced design concept and decades of manufacturing management experience of LEAD-TECH in Australia. To provide first-class experimental equipment for the vast number of technology companies, medical institutions, universities and their potential customers. The products are based on Australian technology and are available in both domestic and imported models. The important parts of domestic assembled models are imported, and the price is between domestic and imported. It fills the contradiction between the quality and price of CO2 incubator in today's market and has a very high cost performance. Lide Taihao (Shanghai) Scientific Instrument Co., Ltd. has independent and perfect after-sales service. Minimize the risk you use. Therefore, the Lead-tech CO2 incubator not only provides an accurate in vitro simulation environment for cell growth, but also makes your cell culture safe and worry-free.
appendix:
Carbon dioxide cell incubator
LT-CIX90FI
LCD touch panel, air sleeve + infrared sensor + HEPA + UV sterilization, 30-segment programming, 99 cycles;
LT-CIX160FI
RT+5°C~65°C
USB/RS232 interface, no condensation technology; CO2 concentration: 0~20Vol%;
LT-CIX250FI
Uniformity: ±0.5 ° C (37 ° C); Volatility: ± 0.1 ° C;
LT-CIX90FT
LCD touch panel, air jacket + thermal conductivity sensor + HEPA + 90 ° C high temperature and high sterilization, 30-segment programming, 99 cycles;
LT-CIX160FT
RT+5°C~65°C
USB interface, no condensation technology; CO2 concentration: 0~20Vol%;
LT-CIX250FT
Uniformity: ±0.5 ° C (37 ° C); Volatility: ± 0.1 ° C;
LT-CIX90WI
LCD touch panel, water jacket + thermal conductivity sensor + HEPA + UV sterilization, 30-stage programming, 99 cycles; external disinfection, configuration CO2 air intake filter.
LT-CIX160WI
RT+5°C~65°C
USB interface, no condensation technology; CO2 concentration: 0~20Vol%;
LT-CIX250WI
Uniformity: ±0.5 ° C (37 ° C); Volatility: ± 0.1 ° C;