The commonly used dilution solution based on sodium citrate and glucose consists of 0.38 g of sodium citrate, 4.54 g of anhydrous glucose, 1 to 3 ml of egg yolk, and 100,000 international units each of penicillin and streptomycin, with distilled water added to make up a total volume of 100 ml. Another option is the sucrose yolk diluent, which contains 11 g of sucrose, 1 to 3 ml of egg yolk, 100,000 international units of both penicillin and streptomycin, and 100 ml of distilled water. A third formulation is the glucose yolk dilution, made by dissolving 7.5 g of anhydrous glucose in 1 to 3 ml of egg yolk, along with 100,000 international units each of penicillin and streptomycin, and then adding distilled water to reach 100 ml. When preparing the diluent, it's essential to use clean, dry containers that have been properly disinfected. Fresh eggs should be used, and all drugs must be pure and of high quality. Accurate measurement of each ingredient is crucial. After dissolving the components, the solution should be filtered and then boiled for 15 to 20 minutes to ensure sterility and proper mixing. When diluting semen, it's important to match the temperature of the diluent with that of the semen to avoid shocking the sperm cells. The diluent should be added slowly along the side of the container holding the semen, not poured directly into it. Reversing this process can cause damage to the sperm and reduce viability. It’s also recommended to use a higher dilution ratio—ideally five times or more—to maintain optimal conditions for sperm survival and activity. Always handle the mixture gently and avoid excessive agitation.

Plant Extract

Tongkat Ali Extract, Maca Root Extract, Naringin Powder, Horny Goat Weed Extract

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