DExD⁄H-box RNA helicase negatively regulates the tolerance of Arabidopsis to low K+

Soil nutrients are important for the growth and metabolism of plants. Plants need to obtain nutrients from the soil and evolve adaptive mechanisms that ensure continued absorption of nutrients under different nutrient conditions. K + is an important nutrient. Under low K + stress, many plants show different degrees of symptoms, such as yellowing of leaves and inhibition of growth. Past studies have found that the gene AKT1, HKT, KT/KUP/HAK family play an important role in K + transit, but the K on how to feel different cell concentrations + do not know much.

In June 2011, scientists at Shandong Agricultural University discovered that the expression of the DExD⁄ H-box RNA helicase gene AtHELPS, a molecular motor protein of Arabidopsis thaliana, was induced by low K + , zeatin and cold treatment, resulting from high K + stress. The expression is lowered. AtHELPS affected the germination and plant weight of Arabidopsis seeds under low K+ conditions. At low K + The levels of AKT1, CBL1⁄9 and CIPK23 mRNA in the helps mutant under stress were higher than those in overexpression. Importantly, the direct determination of K + flow rate NMT technology (the NMT), found K low K + stress helps mutant + influx increased significantly, but the material overexpressed AtHELPS K + flow rate is very low. These results provide information DExD/ H plant box RNA helicase function analysis, we found a low K + plants tolerate negative regulator of coercion.

Now, NMT technology has become an effective tool to study physiological function, in this study, the scientists measured the direct application of NMT Arabidopsis root K + flow rate difference between different genotypes direct map, to explain the function of genes provided Direct evidence.

Key words: DExD ⁄ H-box helicase; deficiency; K + flow rate; seed germination

References: Xu RR, et al . FEBS Journal, 2011, 278: 2296–2306.

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